The hydrogenation of y - linolenic acid by pure cultures of two rumen bacteria
نویسنده
چکیده
Two species of rumen bacteria that have been previously shown to partially hydrogenate a-linolenic acid have been examined for their ability to hydrogenate y-linolenic acid. Free y-linolenic acid is hydrogenated in vitro to stearic acid by a rumen Fusocillus sp. (N.C.I.B. 11026), but only to cis,trans-octadec-6,1 1-enoic acid by a Butyrivibrio sp. The sequential hydrogenations are preceded by a A'2-cis-Al'-trans isomerization identical with that observed in the hydrogenation of a-linolenic acid and linoleic acid.
منابع مشابه
C18 unsaturated fatty acid hydrogenation patterns of some rumen bacteria and their ability to hydrolyse exogenous phospholipid.
1. A number of rumen bacteria isolated because of their ability to deacylate phosphatidyl choline, were found, in addition, to hydrogenate polyunsaturated fatty acids. 2. The most active lipolytic organisms had an unusual pattern of hydrogenation of dietary fatty acids in that alpha-linolenic acid was hydrogenated only as far as trans-11, cis-15-octadecadienoic acid.
متن کاملThe hydrogenation of the series of methylene-interrupted cis,cis-octadecadienoic acids by pure cultures of six rumen bacteria.
The hydrogenation of all the methylene-interrupted cis,cis-octadecadienoic acids was examined using pure cultures of six rumen bacteria able to hydrogenate linoleic acid to stearic acid or its immediate precursor, trans-11-octadecenoic acid, after first conjugating the linoleic acid to cis,trans-9,11-octadecadienoic acid. Only the delta 14-cis,17-cis-isomer was not hydrogenated by at least one ...
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A number of studies have been conducted to elucidate the properties and mechanism of the hydrogenation of Clg unsaturated fatty acids by rumen micro-organisms. Progress in this area has been reviewed by Dawson & Kemp (1970). During screening of a number of pure cultures of rumen bacteria, several species have been shown to hydrogenate linoleate to trans-octadecenoate (Polan, McNeill & Tove, 196...
متن کاملThe biohydrogenation of alpha-linolenic acid and oleic acid by rumen micro-organisms.
1. alpha-[U-(14)C]Linolenic acid was incubated with the rumen contents of sheep and the metabolic products were characterized by thin-layer chromatography, gas-liquid chromatography and absorption spectroscopy in the ultraviolet and infrared. 2. A tentative scheme for the biohydrogenation route to stearic acid is presented. The main pathway is through diconjugated cis-cis-cis-octadecatrienoic a...
متن کاملBiohydrogenation of Unsaturated Fatty Acids by Rumen Bacteria.
Polan, C. E. (North Carolina State of the University of North Carolina, Raleigh), J. J. McNeill, and S. B. Tove. Biohydrogenation of unsaturated fatty acids by rumen bacteria. J. Bacteriol. 88:1056-1064. 1964.-A simple, rapid, specific assay for the biohydrogenation of unsaturated fatty acids was developed. With this assay, it was shown that washed suspensions of mixed rumen bacteria hydrogenat...
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